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10.3390/bioengineering6010022 Bioengineering 9 Di MiccoR.KrizhanovskyV.BakerD.DaddaDi FagagnaF

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The protein was then applied to a Resource Q column (Cytiva) and eluted with a NaCl gradient (50 mM Tris pH 8.0, 01 M NaCl), followed by further purification using a Superose 6 Increase column (Cytiva) in 25 mM Tris pH 8.0, 150 mM NaCl, 1 mM MgCl 2 and 0.5 mM tris(2-carboxyethyl)phosphine (TCEP)