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One of the foremost concerns is the delayed onset of therapeutic effect, often requiring 26 weeks to manifest noticeable clinical improvements, which can be particularly detrimental for patients in acute distress or those at risk of suicide [16, 37, 38]

Immunofluorescence staining was revealed by using a fluorescein-isothiocyanate-conjugated anti-mouse (for Glucagon and HA), fluorescein-isothiocyanate-conjugated anti-rabbit (for pRb S807/811 ), anti-guinea pig (for Insulin) secondary antibodies