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It is generally avoided in people with a personal or family history of medullary thyroid carcinoma or multiple endocrine neoplasia syndrome type 2

2 C ), making it necessary to test whether the effect on water intake was secondary to an effect on food intake

RNA extraction, cDNA synthesis and real-time quantitative PCR (RT-qPCR) Total RNA was isolated from cells using a TRI Reagent kit (Sigma) according to the manufacturers instructions